The derivation of two additional human embryonic stem cell lines from day 3 embryos with low morphological scores

Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos

We report the derivation and characterization of two new human embryonic stem cells (hESC) lines (CU1 and CU2) from embryos with an irreversible loss of integrated organismic function. In addition, we analyzed retrospective data of morphological progression from embryonic day (ED) 5 to ED6 for 2480 embryos not suitable for clinical use to assess grading criteria indicative of loss of viability ...

Derivation of human embryonic stem cell lines.

Human embryonic stem cells (hESC) are undifferentiated cells derived from an early embryo that can grow in vitro indefinitely, while retaining their capability to differentiate into specialized somatic cell types. Over the last decade there has been great interest in derivation and culture of these cells, as they can potentially provide a supply of readily available differentiated cells and tis...

Derivation of embryonic stem-cell lines from human blastocysts.

Embryonic stem cells have the unique ability to form all adult cell types. Harnessing this potential may provide a source of cells to replace those that are lost or impaired as a result of disease. Moreover, the derivation of human embryonic stem cells opens a unique window into the study of early human development. At present, approximately 15 human embryonic stem-cell lines are publicly avail...

Derivation of human embryonic stem cell lines from single cells of 4-cell stage embryos: be aware of the risks.

Sir, Most of the human embryonic stem cell (hESC) lines have been derived from the inner mass of blastocyst stage embryos, some from 8-cell stage morula (Streclchenko et al., 2004) and some from isolated blastomeres from morulae (Chung et al., 2008). In a very recent abstract, a Belgian team (Van de Velde et al., 2008) elegantly established an embryonic stem cell line from a single blastomere i...

Derivation of embryonic stem cell lines.